TY - JOUR T1 - Physical characteristics comparison between maytansinoid-based and auristatin-based antibody-drug conjugates A1 - Fujii, Tomohiro A1 - Reiling, Calliste A1 - Quinn, Colette A1 - Kliman, Michal A1 - Mendelsohn, Brian A A1 - Matsuda, Yutaka Y1 - 2021/// KW - Antibody-drug conjugates KW - hydrophobicity KW - drug-linker KW - conjugation KW - drug-linker JF - Exploration of Targeted Anti-tumor Therapy VL - 2 SP - 576 EP - 585 DO - 10.37349/etat.2021.00064 UR - https://www.explorationpub.com/Journals/etat/Article/100264 N2 - Aim: Direct analytical comparison of two major drug-linkers in the antibody-drug conjugate (ADC) field was conducted. Methods: Four different analytical methods [AlogP calculation, reverse phase (RP) high-performance liquid chromatography (HPLC; RP-HPLC), size exclusion chromatography HPLC (SEC-HPLC), and differential scanning calorimetry (DSC)] were tested for this comparison. Results: Maytansinoid-based ADCs showed less hydrophobicity than auristatin-based ADCs. Regardless of the drug-linker and drug-to-antibody ratios (DARs), the stability detected by DSC was decreased by conjugation. Conclusions: The cost and time-efficient analytical comparison described in this manuscript may be useful information for an initial characterization of ADCs prior to detailed biological studies. ER -