@article{10.37349/eds.2023.00012,
abstract = {Aim: Identification of small bioactive regions in proteins and peptides can be useful information in drug design studies. The current study has shown that an inter-cysteine loop of the N-terminal domain of Opisthorchis viverrini granulin-1 (Ov-GRN-1), a granulin protein from the flatworm liver fluke Opisthorchis viverrini which has potent wound healing properties, maintains the bioactivity of the full-length protein. Methods: Peptides corresponding to the three inter-cysteine loops of the N-terminal domain were produced using synthetic chemistry, and their structures and bioactivities were analyzed using nuclear magnetic resonance (NMR) spectroscopy and cell proliferation assays, respectively. Results: As expected for such small peptides, NMR analysis indicated that the peptides were poorly structured in solution. However, a seven-residue peptide corresponding to loop 2 (GRN-L2) promoted cell proliferation, in contrast to the other fragments. Conclusions: The results from the current study suggest that GRN-L2 might be responsible, in part, for the bioactivity of Ov-GRN-1, and might be a useful lead molecule for subsequent wound healing studies.},
author = {Takjoo, Rozita and Wilson, David T. and Bansal, Paramjit S. and Loukas, Alex and Smout, Michael J. and Daly, Norelle L.},
doi = {10.37349/eds.2023.00012},
journal = {Exploration of Drug Science},
pages = {172--179},
title = {{Identification of an inter-cysteine loop potentially involved in the activity of Opisthorchis viverrini-granulin-1}},
url = {https://www.explorationpub.com/Journals/eds/Article/100812},
volume = {1},
year = {2023},
number = {3}
}