@article{10.37349/ei.2026.1003248,
abstract = {Recurrent infections in children often prompt evaluation for primary immunodeficiency diseases, particularly those affecting humoral immunity. Assessment of memory B cell subsets and vaccine-specific antibody responses provides critical insight into long-term protective immunity. While peripheral blood mononuclear cell (PBMC) isolation is common, direct whole blood staining offers faster processing, reduced cell loss, and better preservation of fragile B cell populations. This comprehensive protocol describes a standardised whole blood flow cytometry method for memory B cell phenotyping, alongside ELISA-based measurement of vaccine-specific antibody titres as part of assessing children with recurrent infections.},
author = {Jamaluddin, Jalilah and Mohamad, Siti Mardhiana and Ismail, Intan Hakimah},
doi = {10.37349/ei.2026.1003248},
journal = {Exploration of Immunology},
elocation-id = {1003248},
title = {Protocol workflow for whole blood memory B cell phenotyping and vaccine-specific antibody quantification},
url = {https://www.explorationpub.com/Journals/ei/Article/1003248},
volume = {6},
year = {2026}
}