@article{Fujii1851,
abstract = {Aim: Direct analytical comparison of two major drug-linkers in the antibody-drug conjugate (ADC) field was conducted.
Methods: Four different analytical methods [AlogP calculation, reverse phase (RP) high-performance liquid chromatography (HPLC; RP-HPLC), size exclusion chromatography HPLC (SEC-HPLC), and differential scanning calorimetry (DSC)] were tested for this comparison.
Results: Maytansinoid-based ADCs showed less hydrophobicity than auristatin-based ADCs. Regardless of the drug-linker and drug-to-antibody ratios (DARs), the stability detected by DSC was decreased by conjugation.
Conclusions: The cost and time-efficient analytical comparison described in this manuscript may be useful information for an initial characterization of ADCs prior to detailed biological studies.}
author = {Fujii, Tomohiro and Reiling, Calliste and Quinn, Colette and Kliman, Michal and Mendelsohn, Brian A and Matsuda, Yutaka},
keywords = {Antibody-drug conjugates, hydrophobicity, drug-linker, conjugation},
pages = {576--585},
title = {{Physical characteristics comparison between maytansinoid-based and auristatin-based antibody-drug conjugates}},
url = {https://www.explorationpub.com/Journals/etat/Article/100264},
volume = {2},
year = {2021}
}