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[Title] => Exploration of biomaterials: a multidisciplinary venture
[Abstract] =>
[AbstractComplete] =>
[Names] => Maryam Tabrizian
[Doi] => 10.37349/ebmx.2023.00001
[Published] => January 01, 2024
[Viewed] => 938
[Downloaded] => 38
[Subject] => Editorial
[Year] => 2024
[CiteUrl] => https://api.crossref.org/works/10.37349/ebmx.2023.00001
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[TitleAbbr] => Explor BioMat-X.
[Pages] => 2024;1:1–4
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[CitethisArticle] => Tabrizian M. Exploration of biomaterials: a multidisciplinary venture. Explor BioMat-X. 2024;1:1–4. https://doi.org/10.37349/ebmx.2023.00001
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[Ris_Time] => 2024-02-23 02:04:35
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[KeysWordContens] => Exploration of biomaterials: a multidisciplinary venture,,,Maryam Tabrizian
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[Title] => Interaction of Pseudomonas aeruginosa with surface-modified silica studied by ultra-high frequency acoustic wave biosensor
[Abstract] => Aim:
This study aimed to examine the amount of surface non-specific adsorption, or fouling, observed by Pseudomonas aeruginosa (P. aeruginosa) on a quartz crystal based acoustic wave biosensor un
[AbstractComplete] =>
Aim:
This study aimed to examine the amount of surface non-specific adsorption, or fouling, observed by Pseudomonas aeruginosa (P. aeruginosa) on a quartz crystal based acoustic wave biosensor under different flow conditions with and without an anti-fouling layer.
Methods:
An electromagnetic piezoelectric acoustic sensor (EMPAS) based on electrode free quartz crystals was used to perform the analysis. Phosphate buffered saline (PBS) was flowed over the crystal surface at various flow rates from 50 μL/min to 200 μL/min, with measurements being taken at the 43rd harmonic (~864 MHz). The crystal was either unmodified, or modified with a monoethylene glycol [2-(3-silylpropyloxy)-hydroxy-ethyl (MEG-OH)] anti-fouling layer. Overnight culture of P. aeruginosa PAO1 (PAO1) in lysogeny broth (LB) was injected into the system, and flow maintained for 30 min.
Results:
The frequency change of the EMPAS crystal after injection of bacteria into the system was found to change based on the flow rate of buffer, suggesting the flow rate has a strong effect on the level of non-specific adsorption. The MEG-OH layer drastically reduced the level of fouling observed under all flow conditions, as well as reduced the amount of variation between experiments. Flow rates of 150 μL/min or higher were found to best reduce the level of fouling observed as well as experimental variation.
Conclusions:
The MEG-OH anti-fouling layer is important for accurate and reproducible biosensing measurements due to the reduced fouling and variation during experiments. Additionally, a flow rate of 150 μL/min may prove better for measurement compared to the current standard of 50 μL/min for this type of instrument.
[Names] => Brian De La Franier, Michael Thompson
[Doi] => 10.37349/ebmx.2023.00002
[Published] => January 01, 2024
[Viewed] => 553
[Downloaded] => 22
[Subject] => Original Article
[Year] => 2024
[CiteUrl] => https://api.crossref.org/works/10.37349/ebmx.2023.00002
[Inline] => 1
[Type] => 1
[Issue] => 1
[Topic] => 0
[TitleAbbr] => Explor BioMat-X.
[Pages] => 2024;1:5–13
[Recommend] => 0
[Keywords] => Anti-fouling, biosensor, acoustic wave,
Pseudomonas aeruginosa
, flow rates
[DetailTitle] =>
[DetailUrl] =>
[Id] => 10132
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[Cited_Time] =>
[CitethisArticle] => De La Franier B, Thompson M. Interaction of Pseudomonas aeruginosa with surface-modified silica studied
by ultra-high frequency acoustic wave biosensor. Explor BioMat-X. 2024;1:5–13. https://doi.org/10.37349/ebmx.2023.00002
[Jindex] => 0
[CName] =>
[CEmail] =>
[Ris_Time] => 2024-02-23 08:30:52
[Bib_Time] => 2024-02-23 08:30:52
[KeysWordContens] => Interaction of Pseudomonas aeruginosa with surface-modified silica studied by ultra-high frequency acoustic wave biosensor, Anti-fouling, biosensor, acoustic wave,
Pseudomonas aeruginosa
, flow rates,
Aim:
This study aimed to examine the amount of surface non-specific adsorption, or fouling, observed by Pseudomonas aeruginosa (P. aeruginosa) on a quartz crystal based acoustic wave biosensor under different flow conditions with and without an anti-fouling layer.
Methods:
An electromagnetic piezoelectric acoustic sensor (EMPAS) based on electrode free quartz crystals was used to perform the analysis. Phosphate buffered saline (PBS) was flowed over the crystal surface at various flow rates from 50 μL/min to 200 μL/min, with measurements being taken at the 43rd harmonic (~864 MHz). The crystal was either unmodified, or modified with a monoethylene glycol [2-(3-silylpropyloxy)-hydroxy-ethyl (MEG-OH)] anti-fouling layer. Overnight culture of P. aeruginosa PAO1 (PAO1) in lysogeny broth (LB) was injected into the system, and flow maintained for 30 min.
Results:
The frequency change of the EMPAS crystal after injection of bacteria into the system was found to change based on the flow rate of buffer, suggesting the flow rate has a strong effect on the level of non-specific adsorption. The MEG-OH layer drastically reduced the level of fouling observed under all flow conditions, as well as reduced the amount of variation between experiments. Flow rates of 150 μL/min or higher were found to best reduce the level of fouling observed as well as experimental variation.
Conclusions:
The MEG-OH anti-fouling layer is important for accurate and reproducible biosensing measurements due to the reduced fouling and variation during experiments. Additionally, a flow rate of 150 μL/min may prove better for measurement compared to the current standard of 50 μL/min for this type of instrument.
,Brian De La Franier, Michael Thompson
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[Title] => Fabrication of anthracite-derived multicolor graphene quantum dots for their potential application in nanomedicine
[Abstract] =>
Aim:
This study aims to discover an alternative precursor with abundant source and low cost for multicolor graphene quantum dots (GQDs) preparation and application.
Methods:
In the current
[AbstractComplete] =>
Aim:
This study aims to discover an alternative precursor with abundant source and low cost for multicolor graphene quantum dots (GQDs) preparation and application.
Methods:
In the current study, anthracite-derived multicolor GQDs were prepared at different reaction temperatures (100°–150°C), referring to the GQDs 100, GQDs 120, GQDs 130, and GQDs 150.
Results:
The GQDs 100, GQDs 120, GQDs 130, and GQDs 150 solutions were found to be orange-red, yellow-green, green, and blue under 365 nm excitation UV (ultraviolet) lamp, respectively. The X-ray photoelectron spectroscopy (XPS) data suggests high temperature intensifies oxidation of the amorphous sp3 carbon, resulting in GQDs with higher crystalline structure (Csp2). Compared with the GQDs 100 and GQDs 120, the GQDs 130 and GQDs 150 showed much better biocompatibility, which may attribute to their higher Csp2 composition and smaller size.
Conclusions:
The results suggest that GQDs 130 and GQDs 150 are ideal candidates for nanomedicine applications, e.g., drug/gene delivery and bio-imaging, etc.
[Names] => Hongyu Pan ... Kai Yu
[Doi] => 10.37349/ebmx.2023.00003
[Published] => January 01, 2024
[Viewed] => 539
[Downloaded] => 19
[Subject] => Original Article
[Year] => 2024
[CiteUrl] => https://api.crossref.org/works/10.37349/ebmx.2023.00003
[Inline] => 1
[Type] => 1
[Issue] => 1
[Topic] => 0
[TitleAbbr] => Explor BioMat-X.
[Pages] => 2024;1:14–22
[Recommend] => 0
[Keywords] => Multicolor graphene quantum dots, anthracite, biocompatibility, bio-imaging
[DetailTitle] =>
[DetailUrl] =>
[Id] => 10133
[ris] => https://www.explorationpub.com/uploads/Article/A10133/3e7d6fefe07b3521c0f6277a015bfeb8.ris
[bib] => https://www.explorationpub.com/uploads/Article/A10133/07320acfe28f9d4c612852c4dc5e699d.bib
[ens] =>
[Cited] => 0
[Cited_Time] =>
[CitethisArticle] => Pan H, Chen J, Zhang W, Chen L, Yoon JY, Li B, et al. Fabrication of anthracite-derived multicolor graphene
quantum dots for their potential application in nanomedicine. Explor BioMat-X. 2024;1:14–22. https://doi.org/10.37349/
ebmx.2023.00003
[Jindex] => 0
[CName] =>
[CEmail] =>
[Ris_Time] => 2024-02-23 08:06:11
[Bib_Time] => 2024-02-23 08:06:11
[KeysWordContens] => Fabrication of anthracite-derived multicolor graphene quantum dots for their potential application in nanomedicine, Multicolor graphene quantum dots, anthracite, biocompatibility, bio-imaging,
Aim:
This study aims to discover an alternative precursor with abundant source and low cost for multicolor graphene quantum dots (GQDs) preparation and application.
Methods:
In the current study, anthracite-derived multicolor GQDs were prepared at different reaction temperatures (100°–150°C), referring to the GQDs 100, GQDs 120, GQDs 130, and GQDs 150.
Results:
The GQDs 100, GQDs 120, GQDs 130, and GQDs 150 solutions were found to be orange-red, yellow-green, green, and blue under 365 nm excitation UV (ultraviolet) lamp, respectively. The X-ray photoelectron spectroscopy (XPS) data suggests high temperature intensifies oxidation of the amorphous sp3 carbon, resulting in GQDs with higher crystalline structure (Csp2). Compared with the GQDs 100 and GQDs 120, the GQDs 130 and GQDs 150 showed much better biocompatibility, which may attribute to their higher Csp2 composition and smaller size.
Conclusions:
The results suggest that GQDs 130 and GQDs 150 are ideal candidates for nanomedicine applications, e.g., drug/gene delivery and bio-imaging, etc.
,Hongyu Pan ... Kai Yu
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[Title] => Journey of medical device development from the bench to the bedside—four real-life examples of commercial biomaterials development
[Abstract] => Translating biomaterials research into clinical products is a multidisciplinary yet rewarding journey. It should primarily aim to improve patient treatment and clinical outcomes by addressing unmet
[AbstractComplete] =>
Translating biomaterials research into clinical products is a multidisciplinary yet rewarding journey. It should primarily aim to improve patient treatment and clinical outcomes by addressing unmet clinical needs. Four examples of commercial development of biomaterial implants illustrate the diversity of paths, starting from academic research work (AlchiMedics, TISSIUM, Cousin Surgery) or corporate initiatives to develop new products (Medtronic-Sofradim Production). They have been selected from the Translational Research session of the 2022 Conference of the European Society for Biomaterials (ESB) in Bordeaux (France). Commitment, agility, and perseverance were among the key common skills to successfully meet challenges, especially the most unexpected ones. All dimensions of translation projects must be integrated from the start, including the regulatory strategy.
[Names] => Yves Bayon ... Didier Letourneur
[Doi] => 10.37349/ebmx.2024.00004
[Published] => January 16, 2024
[Viewed] => 658
[Downloaded] => 39
[Subject] => Review
[Year] => 2024
[CiteUrl] => https://api.crossref.org/works/10.37349/ebmx.2023.00004
[Inline] => 1
[Type] => 1
[Issue] => 1
[Topic] => 0
[TitleAbbr] => Explor BioMat-X.
[Pages] => 2024;1:23–33
[Recommend] => 0
[Keywords] => Biomaterials, medical devices, translation, clinical development
[DetailTitle] =>
[DetailUrl] =>
[Id] => 10134
[ris] => https://www.explorationpub.com/uploads/Article/A10134/982e8fba1fc319049b95ceddaef28a8a.ris
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[ens] =>
[Cited] => 0
[Cited_Time] =>
[CitethisArticle] => Bayon Y, Bureau C, Perreira M, Aubert F, Caillibotte M, Vestberg R, et al. Journey of medical device development from the bench to the bedside—four real-life examples of commercial biomaterials development. Explor BioMat-X. 2024;1:23–33. https://doi.org/10.37349/ebmx.2024.00004
[Jindex] => 0
[CName] =>
[CEmail] =>
[Ris_Time] => 2024-01-16 01:17:48
[Bib_Time] => 2024-01-16 01:17:48
[KeysWordContens] => Journey of medical device development from the bench to the bedside—four real-life examples of commercial biomaterials development, Biomaterials, medical devices, translation, clinical development,
Translating biomaterials research into clinical products is a multidisciplinary yet rewarding journey. It should primarily aim to improve patient treatment and clinical outcomes by addressing unmet clinical needs. Four examples of commercial development of biomaterial implants illustrate the diversity of paths, starting from academic research work (AlchiMedics, TISSIUM, Cousin Surgery) or corporate initiatives to develop new products (Medtronic-Sofradim Production). They have been selected from the Translational Research session of the 2022 Conference of the European Society for Biomaterials (ESB) in Bordeaux (France). Commitment, agility, and perseverance were among the key common skills to successfully meet challenges, especially the most unexpected ones. All dimensions of translation projects must be integrated from the start, including the regulatory strategy.
,Yves Bayon ... Didier Letourneur
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[Title] => Fabrication and characterization of pHEMA hydrogel conduit containing GelMA-HaMA IPN for peripheral nerve regeneration
[Abstract] => Aim:
Small defects after any injury to the periperal nerves results in self-regeneration. However, for larger defects, suturing or grafting are necessary, which may have limitations. Thus, resear
[AbstractComplete] =>
Aim:
Small defects after any injury to the periperal nerves results in self-regeneration. However, for larger defects, suturing or grafting are necessary, which may have limitations. Thus, research on nerve guidence conduits is needed without drawbacks. The aim of the study was to develop hydrogel-based conduits containing interpenetrating network (IPN).
Methods:
Methacrylated gelatin (GelMA)-methacrylated hyaluronic acid (HaMA) IPN was filled the poly(2-hydroxyethylmethacrylate) (pHEMA) the outer conduit. Schwann cells (SCs) were used on the pHEMA and the distal end of the tube was injected with netrin-1 to support model SH-SY5Y cells.
Results:
1H-nuclear magnetic resonance (1H-NMR) showed that methacrylation degrees were 94% ± 2% for GelMA and 60% ± 7% for HaMA. The fraction of HaMA increased the degradation rate; pure HaMA degraded in 3 weeks, while pure GelMA in more than 5 weeks. An increase in the fraction of 2-hydroxyethylmethacrylate (HEMA) from 20% to 56% decreased the porosity and the pore size, significantly. SH-SY5Y cells migrated along the conduit in the presence of netrin-1. NeuN expression was increased in 2 weeks indicating neuronal activity.
Conclusions:
SH-SY5Y cells produced neurites in the IPN. pHEMA conduit including GelMA-HaMA IPN is a good candidate for peripheral nerve regeneration applications. As future studies, the conduit will be tested in vivo for nerve regeneration.
[Names] => Damla Arslantunali Sahin ... Vasif Hasirci
[Doi] => 10.37349/ebmx.2024.00005
[Published] => February 26, 2024
[Viewed] => 507
[Downloaded] => 21
[Subject] => Original Article
[Year] => 2024
[CiteUrl] => https://api.crossref.org/works/10.37349/ebmx.2024.00005
[Inline] => 1
[Type] => 1
[Issue] => 1
[Topic] => 204
[TitleAbbr] => Explor BioMat-X.
[Pages] => 2024;1:34–57
[Recommend] => 0
[Keywords] => Peripheral nerve injury, axon regeneration, nerve guidance conduit, hydrogel, poly(2-hydroxyethylmethacrylate), methacrylated gelatin, hyaluronic acid, interpenetrating network
[DetailTitle] => Nature-Based Biomaterials for Biomedical Applications
[DetailUrl] => https://www.explorationpub.com/Journals/ebmx/Special_Issues/204
[Id] => 10135
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[ens] =>
[Cited] => 0
[Cited_Time] =>
[CitethisArticle] => Sahin DA, Son CD, Hasirci V. Fabrication and characterization of pHEMA hydrogel conduit containing GelMA-HaMA IPN for peripheral nerve regeneration. Explor BioMat-X. 2024;1:34–57. https://doi.org/10.37349/ebmx.2024.00005
[Jindex] => 0
[CName] =>
[CEmail] =>
[Ris_Time] => 2024-02-26 08:26:52
[Bib_Time] => 2024-02-26 08:26:52
[KeysWordContens] => Fabrication and characterization of pHEMA hydrogel conduit containing GelMA-HaMA IPN for peripheral nerve regeneration, Peripheral nerve injury, axon regeneration, nerve guidance conduit, hydrogel, poly(2-hydroxyethylmethacrylate), methacrylated gelatin, hyaluronic acid, interpenetrating network,
Aim:
Small defects after any injury to the periperal nerves results in self-regeneration. However, for larger defects, suturing or grafting are necessary, which may have limitations. Thus, research on nerve guidence conduits is needed without drawbacks. The aim of the study was to develop hydrogel-based conduits containing interpenetrating network (IPN).
Methods:
Methacrylated gelatin (GelMA)-methacrylated hyaluronic acid (HaMA) IPN was filled the poly(2-hydroxyethylmethacrylate) (pHEMA) the outer conduit. Schwann cells (SCs) were used on the pHEMA and the distal end of the tube was injected with netrin-1 to support model SH-SY5Y cells.
Results:
1H-nuclear magnetic resonance (1H-NMR) showed that methacrylation degrees were 94% ± 2% for GelMA and 60% ± 7% for HaMA. The fraction of HaMA increased the degradation rate; pure HaMA degraded in 3 weeks, while pure GelMA in more than 5 weeks. An increase in the fraction of 2-hydroxyethylmethacrylate (HEMA) from 20% to 56% decreased the porosity and the pore size, significantly. SH-SY5Y cells migrated along the conduit in the presence of netrin-1. NeuN expression was increased in 2 weeks indicating neuronal activity.
Conclusions:
SH-SY5Y cells produced neurites in the IPN. pHEMA conduit including GelMA-HaMA IPN is a good candidate for peripheral nerve regeneration applications. As future studies, the conduit will be tested in vivo for nerve regeneration.
,Damla Arslantunali Sahin ... Vasif Hasirci
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[Title] => A biomimetic approach to modulating the sustained release of fibroblast growth factor 2 from fibrin microthread scaffolds
[Abstract] =>
Aim:
The pleiotropic effect of fibroblast growth factor 2 (FGF2) on promoting myogenesis, angiogenesis, and innervation makes it an ideal growth factor for treating volumetric muscle loss (VML) i
[AbstractComplete] =>
Aim:
The pleiotropic effect of fibroblast growth factor 2 (FGF2) on promoting myogenesis, angiogenesis, and innervation makes it an ideal growth factor for treating volumetric muscle loss (VML) injuries. While an initial delivery of FGF2 has demonstrated enhanced regenerative potential, the sustained delivery of FGF2 from scaffolds with robust structural properties as well as biophysical and biochemical signaling cues has yet to be explored for treating VML. The goal of this study is to develop an instructive fibrin microthread scaffold with intrinsic topographic alignment cues as well as regenerative signaling cues and a physiologically relevant, sustained release of FGF2 to direct myogenesis and ultimately enhance functional muscle regeneration.
Methods:
Heparin was passively adsorbed or carbodiimide-conjugated to microthreads, creating a biomimetic binding strategy, mimicking FGF2 sequestration in the extracellular matrix (ECM). It was also evaluated whether FGF2 incorporated into fibrin microthreads would yield sustained release. It was hypothesized that heparin-conjugated and co-incorporated (co-inc) fibrin microthreads would facilitate sustained release of FGF2 from the scaffold and enhance in vitro myoblast proliferation and outgrowth.
Results:
Toluidine blue staining and Fourier transform infrared spectroscopy confirmed that carbodiimide-conjugated heparin bound to fibrin microthreads in a dose-dependent manner. Release kinetics revealed that heparin-conjugated fibrin microthreads exhibited sustained release of FGF2 over a period of one week. An in vitro assay demonstrated that FGF2 released from microthreads remained bioactive, stimulating myoblast proliferation over four days. Finally, a cellular outgrowth assay suggests that FGF2 promotes increased outgrowth onto microthreads.
Conclusions:
It was anticipated that the combined effects of fibrin microthread structural properties, topographic alignment cues, and FGF2 release profiles will facilitate the fabrication of a biomimetic scaffold that enhances the regeneration of functional muscle tissue for the treatment of VML injuries.
[Names] => Meagan E. Carnes ... George D. Pins
[Doi] => 10.37349/ebmx.2024.00006
[Published] => April 23, 2024
[Viewed] => 142
[Downloaded] => 8
[Subject] => Original Article
[Year] => 2024
[CiteUrl] => https://api.crossref.org/works/10.37349/ebmx.2024.00006
[Inline] => 1
[Type] => 1
[Issue] => 2
[Topic] => 0
[TitleAbbr] => Explor BioMat-X.
[Pages] => 2024;1:58–83
[Recommend] => 0
[Keywords] => Fibroblast growth factor 2, fibrin microthreads, fibrin, tissue engineering, skeletal muscle, myoblast
[DetailTitle] =>
[DetailUrl] =>
[Id] => 10136
[ris] => https://www.explorationpub.com/uploads/Article/A10136/fef60538c40842c994ff074075b28b6f.ris
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[ens] =>
[Cited] => 0
[Cited_Time] =>
[CitethisArticle] => Carnes ME, Gonyea CR, Coburn JM, Pins GD. A biomimetic approach to modulating the sustained release of fibroblast growth factor 2 from fibrin microthread scaffolds. Explor BioMat-X. 2024;1:58–83. https://doi.org/10.37349/ebmx.2024.00006
[Jindex] => 0
[CName] =>
[CEmail] =>
[Ris_Time] => 2024-04-23 08:21:00
[Bib_Time] => 2024-04-23 08:21:00
[KeysWordContens] => A biomimetic approach to modulating the sustained release of fibroblast growth factor 2 from fibrin microthread scaffolds, Fibroblast growth factor 2, fibrin microthreads, fibrin, tissue engineering, skeletal muscle, myoblast,
Aim:
The pleiotropic effect of fibroblast growth factor 2 (FGF2) on promoting myogenesis, angiogenesis, and innervation makes it an ideal growth factor for treating volumetric muscle loss (VML) injuries. While an initial delivery of FGF2 has demonstrated enhanced regenerative potential, the sustained delivery of FGF2 from scaffolds with robust structural properties as well as biophysical and biochemical signaling cues has yet to be explored for treating VML. The goal of this study is to develop an instructive fibrin microthread scaffold with intrinsic topographic alignment cues as well as regenerative signaling cues and a physiologically relevant, sustained release of FGF2 to direct myogenesis and ultimately enhance functional muscle regeneration.
Methods:
Heparin was passively adsorbed or carbodiimide-conjugated to microthreads, creating a biomimetic binding strategy, mimicking FGF2 sequestration in the extracellular matrix (ECM). It was also evaluated whether FGF2 incorporated into fibrin microthreads would yield sustained release. It was hypothesized that heparin-conjugated and co-incorporated (co-inc) fibrin microthreads would facilitate sustained release of FGF2 from the scaffold and enhance in vitro myoblast proliferation and outgrowth.
Results:
Toluidine blue staining and Fourier transform infrared spectroscopy confirmed that carbodiimide-conjugated heparin bound to fibrin microthreads in a dose-dependent manner. Release kinetics revealed that heparin-conjugated fibrin microthreads exhibited sustained release of FGF2 over a period of one week. An in vitro assay demonstrated that FGF2 released from microthreads remained bioactive, stimulating myoblast proliferation over four days. Finally, a cellular outgrowth assay suggests that FGF2 promotes increased outgrowth onto microthreads.
Conclusions:
It was anticipated that the combined effects of fibrin microthread structural properties, topographic alignment cues, and FGF2 release profiles will facilitate the fabrication of a biomimetic scaffold that enhances the regeneration of functional muscle tissue for the treatment of VML injuries.
,Meagan E. Carnes ... George D. Pins
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[Title] => Effects of cold plasma treatment on the biological performances of decellularized bovine pericardium extracellular matrix-based films for biomedical applications
[Abstract] =>
Aim:
Since decades, decellularized extracellular matrix (dECM)-derived materials have received worldwide attention as promising biomaterials for tissue engineering and biomedical applications. So
[AbstractComplete] =>
Aim:
Since decades, decellularized extracellular matrix (dECM)-derived materials have received worldwide attention as promising biomaterials for tissue engineering and biomedical applications. Soluble dECM is a versatile raw material that can be easily engineered into the desired shapes and structures. However, there are still some limitations restricting its use, including low hydrophilicity and smooth surfaces, which negatively influence cell adhesion/spreading. The objective of the present study was to investigate surface modification by nitrogen/hydrogen (N2/H2) low-pressure cold plasma treatment as a potential technique to improve the biological response of bovine pericardium dECM films.
Methods:
Bovine pericardium dECM was enzymatically digested and lyophilized prior to the preparation of thin films via solvent-casting method. Changes in surface properties after plasma treatment were investigated using water contact angle (WCA) and X-ray photoelectron spectroscopy (XPS) measurements. Immunofluorescence staining and resazurin assay for human dermal fibroblasts (HDFs) cultured on the dECM films were used to assess the bioactivity of dECM films. Finally, the hemocompatibility of the films was investigated via clotting time and hemolysis assay.
Results:
WCA and XPS results revealed that oxygen (O)- and N-containing functional groups were incorporated onto the film surface and an increase in hydrophilicity was observed after plasma treatment. In vitro experiments showed that cell adhesion in plasma-treated dECM films is much faster if compared to the untreated controls. Moreover, the fibroblast proliferation increased after plasma surface modifications. Finally, the hemocompatibility analysis results indicated a delayed blood clotting and no hemolytic effects for all the tested samples.
Conclusions:
These findings confirmed the potential of dECM as raw material for biocompatible thin films fabrication. Additionally, plasma surface treatment emerged as an eco-friendly and cost-effective strategy to enhance in vitro cell attachment and proliferation on dECM films, expanding their applications in biomedicine.
[Names] => Maria Elena Lombardo ... Diego Mantovani
[Doi] => 10.37349/ebmx.2024.00007
[Published] => April 23, 2024
[Viewed] => 179
[Downloaded] => 11
[Subject] => Original Article
[Year] => 2024
[CiteUrl] => https://api.crossref.org/works/10.37349/ebmx.2024.00007
[Inline] => 1
[Type] => 1
[Issue] => 2
[Topic] => 0
[TitleAbbr] => Explor BioMat-X.
[Pages] => 2024;1:84–99
[Recommend] => 0
[Keywords] => Natural polymers, decellularized extracellular matrix, functionalization, low-pressure cold plasma, surface modifications, physicochemical properties, cell adhesion, hemocompatibility
[DetailTitle] =>
[DetailUrl] =>
[Id] => 10137
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[ens] =>
[Cited] => 0
[Cited_Time] =>
[CitethisArticle] => Lombardo ME, Mariscotti V, Chevallier P, Copes F, Boccafoschi F, Sarkissian A, et al. Effects of cold plasma
treatment on the biological performances of decellularized bovine pericardium extracellular matrix-based films for
biomedical applications. Explor BioMat-X. 2024;1:84–99. https://doi.org/10.37349/ebmx.2024.00007
[Jindex] => 0
[CName] =>
[CEmail] =>
[Ris_Time] => 2024-04-15 07:56:08
[Bib_Time] => 2024-04-15 07:56:08
[KeysWordContens] => Effects of cold plasma treatment on the biological performances of decellularized bovine pericardium extracellular matrix-based films for biomedical applications, Natural polymers, decellularized extracellular matrix, functionalization, low-pressure cold plasma, surface modifications, physicochemical properties, cell adhesion, hemocompatibility,
Aim:
Since decades, decellularized extracellular matrix (dECM)-derived materials have received worldwide attention as promising biomaterials for tissue engineering and biomedical applications. Soluble dECM is a versatile raw material that can be easily engineered into the desired shapes and structures. However, there are still some limitations restricting its use, including low hydrophilicity and smooth surfaces, which negatively influence cell adhesion/spreading. The objective of the present study was to investigate surface modification by nitrogen/hydrogen (N2/H2) low-pressure cold plasma treatment as a potential technique to improve the biological response of bovine pericardium dECM films.
Methods:
Bovine pericardium dECM was enzymatically digested and lyophilized prior to the preparation of thin films via solvent-casting method. Changes in surface properties after plasma treatment were investigated using water contact angle (WCA) and X-ray photoelectron spectroscopy (XPS) measurements. Immunofluorescence staining and resazurin assay for human dermal fibroblasts (HDFs) cultured on the dECM films were used to assess the bioactivity of dECM films. Finally, the hemocompatibility of the films was investigated via clotting time and hemolysis assay.
Results:
WCA and XPS results revealed that oxygen (O)- and N-containing functional groups were incorporated onto the film surface and an increase in hydrophilicity was observed after plasma treatment. In vitro experiments showed that cell adhesion in plasma-treated dECM films is much faster if compared to the untreated controls. Moreover, the fibroblast proliferation increased after plasma surface modifications. Finally, the hemocompatibility analysis results indicated a delayed blood clotting and no hemolytic effects for all the tested samples.
Conclusions:
These findings confirmed the potential of dECM as raw material for biocompatible thin films fabrication. Additionally, plasma surface treatment emerged as an eco-friendly and cost-effective strategy to enhance in vitro cell attachment and proliferation on dECM films, expanding their applications in biomedicine.
,Maria Elena Lombardo ... Diego Mantovani
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[Title] => Unlocking the potential of circulating small extracellular vesicles in neurodegenerative disease through targeted biomarkers and advancements in biosensing
[Abstract] => Neurodegenerative diseases (NDDs) gradually affect neurons impacting both their function and structure, and they afflict millions worldwide. Detecting these conditions before symptoms arise is cruci
[AbstractComplete] =>
Neurodegenerative diseases (NDDs) gradually affect neurons impacting both their function and structure, and they afflict millions worldwide. Detecting these conditions before symptoms arise is crucial for better prognosis and duality of life, given that the disease processes often begin years earlier. Yet, reliable and affordable methods to diagnose NDDs in these stages are currently lacking. There’s a growing interest in using circulating extracellular vesicles (EVs), like small EVs (sEVs) also known as exosomes, as potential sources of markers for screening, diagnosing, and monitoring NDDs. This interest stems from evidence showing that these EVs can carry brain pathological proteins implicated in NDD pathology, and they can even traverse the blood-brain barrier. This review focuses on the creation of EVs, particularly sEVs with a size of less than 200 nanometers, methods for isolating sEVs, and recent advancements in biosensor development to detect NDD-related markers found in sEVs. Furthermore, it explores the potential of sEVs in diagnosing four major NDDs: Alzheimer’s disease (AD), Parkinson’s disease (PD), Huntington’s disease (HD), and multiple system atrophy (MSA).
[Names] => Saqer Al Abdullah ... Kristen Dellinger
[Doi] => 10.37349/ebmx.2024.00008
[Published] => April 24, 2024
[Viewed] => 241
[Downloaded] => 12
[Subject] => Review
[Year] => 2024
[CiteUrl] => https://api.crossref.org/works/10.37349/ebmx.2024.00008
[Inline] => 1
[Type] => 1
[Issue] => 2
[Topic] => 0
[TitleAbbr] => Explor BioMat-X.
[Pages] => 2024;1:100–123
[Recommend] => 0
[Keywords] => Exosomes, biosensing, Alzheimer’s disease, Parkinson’s disease
[DetailTitle] =>
[DetailUrl] =>
[Id] => 10138
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[ens] =>
[Cited] => 0
[Cited_Time] =>
[CitethisArticle] => Al Abdullah S, Cocklereece I, Dellinger K. Unlocking the potential of circulating small extracellular vesicles in neurodegenerative disease through targeted biomarkers and advancements in biosensing. Explor BioMat-X. 2024;1:100–23. https://doi.org/10.37349/ebmx.2024.00008
[Jindex] => 0
[CName] =>
[CEmail] =>
[Ris_Time] => 2024-04-16 00:57:01
[Bib_Time] => 2024-04-16 00:57:01
[KeysWordContens] => Unlocking the potential of circulating small extracellular vesicles in neurodegenerative disease through targeted biomarkers and advancements in biosensing, Exosomes, biosensing, Alzheimer’s disease, Parkinson’s disease,
Neurodegenerative diseases (NDDs) gradually affect neurons impacting both their function and structure, and they afflict millions worldwide. Detecting these conditions before symptoms arise is crucial for better prognosis and duality of life, given that the disease processes often begin years earlier. Yet, reliable and affordable methods to diagnose NDDs in these stages are currently lacking. There’s a growing interest in using circulating extracellular vesicles (EVs), like small EVs (sEVs) also known as exosomes, as potential sources of markers for screening, diagnosing, and monitoring NDDs. This interest stems from evidence showing that these EVs can carry brain pathological proteins implicated in NDD pathology, and they can even traverse the blood-brain barrier. This review focuses on the creation of EVs, particularly sEVs with a size of less than 200 nanometers, methods for isolating sEVs, and recent advancements in biosensor development to detect NDD-related markers found in sEVs. Furthermore, it explores the potential of sEVs in diagnosing four major NDDs: Alzheimer’s disease (AD), Parkinson’s disease (PD), Huntington’s disease (HD), and multiple system atrophy (MSA).
,Saqer Al Abdullah ... Kristen Dellinger
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[coverUrl] => https://www.explorationpub.com/uploads/Article/A10139/10139-cover.png
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[Title] => Navigating regulatory challenges in molecularly tailored nanomedicine
[Abstract] => Nanomedicine, a convergence of nanotechnology and medical sciences, has unleashed transformative potential in healthcare. However, harnessing the benefits of nanomedicine requires a thorough underst
[AbstractComplete] =>
Nanomedicine, a convergence of nanotechnology and medical sciences, has unleashed transformative potential in healthcare. However, harnessing the benefits of nanomedicine requires a thorough understanding of its regulatory landscape. An in-depth discussion of regulatory considerations, including molecular safety assessment, harmonization of the regulatory landscape, and shaping the future of innovation, is presented in this discourse. The molecular safety assessment entails evaluating interactions between nanoparticles and biomolecules, ensuring compatibility at the molecular level. Harmonization involves developing international standards and guidelines for a consistent regulatory approach, while shaping innovations emphasizes integrating molecular safety assessments into early stages of development. Challenges encompass the need for standardized assessment methods, balancing innovation with safety, and addressing unique features of novel molecular designs. As the nanomedicine landscape evolves, effective regulatory strategies must navigate the intricate interplay of molecules and technologies, ensuring both patient access and product safety.
[Names] => Ajay Vikram Singh ... Paolo Zamboni
[Doi] => 10.37349/ebmx.2024.00009
[Published] => April 25, 2024
[Viewed] => 220
[Downloaded] => 9
[Subject] => Commentary
[Year] => 2024
[CiteUrl] => https://api.crossref.org/works/10.37349/ebmx.2024.00009
[Inline] => 1
[Type] => 1
[Issue] => 2
[Topic] => 0
[TitleAbbr] => Explor BioMat-X.
[Pages] => 2024;1:124–134
[Recommend] => 0
[Keywords] => Nanomedicine, regulatory considerations, molecular safety assessment, harmonization, innovations, patient safety
[DetailTitle] =>
[DetailUrl] =>
[Id] => 10139
[ris] => https://www.explorationpub.com/uploads/Article/A10139/a48d98fea657c48520c3b7369796403b.ris
[bib] => https://www.explorationpub.com/uploads/Article/A10139/271f56d4e1205f2e2f0b14a2f62ec760.bib
[ens] =>
[Cited] => 0
[Cited_Time] =>
[CitethisArticle] => Singh AV, Bhardwaj P, Upadhyay AK, Pagani A, Upadhyay J, Bhadra J, et al. Navigating regulatory challenges in molecularly tailored nanomedicine. Explor BioMat-X. 2024;1:124–34. https://doi.org/10.37349/ebmx.2024.00009
[Jindex] => 0
[CName] =>
[CEmail] =>
[Ris_Time] => 2024-04-25 08:15:31
[Bib_Time] => 2024-04-17 03:21:16
[KeysWordContens] => Navigating regulatory challenges in molecularly tailored nanomedicine, Nanomedicine, regulatory considerations, molecular safety assessment, harmonization, innovations, patient safety,
Nanomedicine, a convergence of nanotechnology and medical sciences, has unleashed transformative potential in healthcare. However, harnessing the benefits of nanomedicine requires a thorough understanding of its regulatory landscape. An in-depth discussion of regulatory considerations, including molecular safety assessment, harmonization of the regulatory landscape, and shaping the future of innovation, is presented in this discourse. The molecular safety assessment entails evaluating interactions between nanoparticles and biomolecules, ensuring compatibility at the molecular level. Harmonization involves developing international standards and guidelines for a consistent regulatory approach, while shaping innovations emphasizes integrating molecular safety assessments into early stages of development. Challenges encompass the need for standardized assessment methods, balancing innovation with safety, and addressing unique features of novel molecular designs. As the nanomedicine landscape evolves, effective regulatory strategies must navigate the intricate interplay of molecules and technologies, ensuring both patient access and product safety.
,Ajay Vikram Singh ... Paolo Zamboni
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[Title] => Nanoparticle-functionalized acrylic bone cement for local therapeutic delivery to spine metastases
[Abstract] =>
Aim:
Polymethylmethacrylate bone cement is often used to reconstruct critical-sized defects generated by the surgical resection of spinal metastases. Residual tumor cells after a resection can dr
[AbstractComplete] =>
Aim:
Polymethylmethacrylate bone cement is often used to reconstruct critical-sized defects generated by the surgical resection of spinal metastases. Residual tumor cells after a resection can drive recurrence and destabilization. Doxorubicin (DOX) is a common chemotherapeutic drug with unwanted side-effects when administered systemically. Mesoporous silica nanoparticles (NPs) are gaining attention for targeted drug delivery to bypass the negative side effects associated with systemic drug administration. An NP-functionalized cement was developed for the local release of DOX and its ability to suppress cancer cells was tested.
Methods:
DOX was loaded onto NPs which were then mixed into the cement. Static contact angles were measured. Drug release profiles were obtained over a period of 4 weeks. Cement constructs were incubated with two-dimensional (2D) cultures of human bone-marrow derived mesenchymal stem cells and human osteoblasts, as well as 2D and three-dimensional (3D) cultures of breast and prostate cancer cell lines. Cell metabolic activity and viability were evaluated. Cell migration and spheroid growth of cancer cell lines were assessed in collagen-coated spheroid cultures.
Results:
NPs were homogenously dispersed and did not alter the mechanical strength nor the wettability of the cement. A sustained DOX release profile was achieved with the addition of NPs to the bone cement. The release profile of DOX from NP cement may be modified by varying the amount of the drug loaded onto the NPs and the proportion of NPs in the cement. Cancer cells treated with the cement constructs showed a dose- and time-dependent inhibition, with minimal toxicity against healthy cells. Cancer cell migration and spheroid growth were impaired in 3D culture.
Conclusions:
NPs were shown to be essential for sustained DOX release from bone cement. DOX-loaded NP cement can inhibit cancer cells and impair their migration, with strong potential for in vivo translation studies.
[Names] => Ateeque Siddique ... Derek H. Rosenzweig
[Doi] => 10.37349/ebmx.2024.00010
[Published] => April 28, 2024
[Viewed] => 171
[Downloaded] => 8
[Subject] => Original Article
[Year] => 2024
[CiteUrl] => https://api.crossref.org/works/10.37349/ebmx.2024.00010
[Inline] => 1
[Type] => 1
[Issue] => 2
[Topic] => 0
[TitleAbbr] => Explor BioMat-X.
[Pages] => 2024;1:135–157
[Recommend] => 0
[Keywords] => Polymethylmethacrylate, mesoporous silica nanoparticles, chemotherapeutics, local delivery, three-dimensional culture
[DetailTitle] =>
[DetailUrl] =>
[Id] => 101310
[ris] => https://www.explorationpub.com/uploads/Article/A101310/8cbe9acf6f0352d9d7aa3849bac96cd1.ris
[bib] => https://www.explorationpub.com/uploads/Article/A101310/6a8f8220773dc8385a1d543def583c6b.bib
[ens] =>
[Cited] => 0
[Cited_Time] =>
[CitethisArticle] => Siddique A, Garakani MM, Cooke ME, Weber MH, Rosenzweig DH. Nanoparticle-functionalized acrylic bone cement for local therapeutic delivery to spine metastases. Explor BioMat-X. 2024;1:135–57. https://doi.org/10.37349/ebmx.2024.00010
[Jindex] => 0
[CName] =>
[CEmail] =>
[Ris_Time] => 2024-04-23 06:32:57
[Bib_Time] => 2024-04-23 06:32:57
[KeysWordContens] => Nanoparticle-functionalized acrylic bone cement for local therapeutic delivery to spine metastases, Polymethylmethacrylate, mesoporous silica nanoparticles, chemotherapeutics, local delivery, three-dimensional culture,
Aim:
Polymethylmethacrylate bone cement is often used to reconstruct critical-sized defects generated by the surgical resection of spinal metastases. Residual tumor cells after a resection can drive recurrence and destabilization. Doxorubicin (DOX) is a common chemotherapeutic drug with unwanted side-effects when administered systemically. Mesoporous silica nanoparticles (NPs) are gaining attention for targeted drug delivery to bypass the negative side effects associated with systemic drug administration. An NP-functionalized cement was developed for the local release of DOX and its ability to suppress cancer cells was tested.
Methods:
DOX was loaded onto NPs which were then mixed into the cement. Static contact angles were measured. Drug release profiles were obtained over a period of 4 weeks. Cement constructs were incubated with two-dimensional (2D) cultures of human bone-marrow derived mesenchymal stem cells and human osteoblasts, as well as 2D and three-dimensional (3D) cultures of breast and prostate cancer cell lines. Cell metabolic activity and viability were evaluated. Cell migration and spheroid growth of cancer cell lines were assessed in collagen-coated spheroid cultures.
Results:
NPs were homogenously dispersed and did not alter the mechanical strength nor the wettability of the cement. A sustained DOX release profile was achieved with the addition of NPs to the bone cement. The release profile of DOX from NP cement may be modified by varying the amount of the drug loaded onto the NPs and the proportion of NPs in the cement. Cancer cells treated with the cement constructs showed a dose- and time-dependent inhibition, with minimal toxicity against healthy cells. Cancer cell migration and spheroid growth were impaired in 3D culture.
Conclusions:
NPs were shown to be essential for sustained DOX release from bone cement. DOX-loaded NP cement can inhibit cancer cells and impair their migration, with strong potential for in vivo translation studies.
,Ateeque Siddique ... Derek H. Rosenzweig
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