Metabolic dysfunction-associated steatotic liver disease (MASLD), formerly known as nonalcoholic fatty liver disease, defines steatotic liver disease (SLD) in the presence of at least one cardiometabolic risk factor without any harmful alcohol intake [1]. The MASLD spectrum comprises simple steatosis, metabolic dysfunction-associated steatohepatitis [(MASH), previously known as nonalcoholic steatohepatitis (NASH)], fibrosis, cirrhosis with or without hepatocellular carcinoma [1, 2]. Collectively, MASLD poses a heavy clinical and epidemiological burden against which limited therapeutic strategies are available [2–4]. The pathogenesis of MASLD is complex and multi-factorial, with various noxious stimuli interacting in parallel to contribute to liver injury in the context of systemic metabolic dysfunction [5]. Among such noxious stimuli, type 2 diabetes (T2D) plays a major role given that T2D facilitates development and progression of MASLD and that MASLD, in turn, worsens glucose homeostasis [6]. Therefore, MASLD is a systemic condition and requires a holistic approach [4, 7]. Sex differences and liver fibrosis are major determinants of the whole spectrum of hepatic and extrahepatic manifestations of MASLD [4, 8, 9], accounting for the importance in this field of sex-specific analysis of data and of animal models faithfully recapitulating fibrosing disease such as seen in human disease.

Various organs comprising not only the liver but several extrahepatic organs such as the pancreas and the adipose tissue, kidneys, the skeletal muscle, and duodenum regulate metabolic physiology and participate in the development of systemic metabolic dysregulation. Among these, the duodenum is not universally appreciated as a key metabolic organ. Instead, several lines of evidence fully support its role in the physiopathology of metabolism. Evidence has shown that prolonged fasting will induce atrophy of duodenal mucosa with severe ultrastructural changes in a proportion of cases [10] and that, conversely, obesogenic diets rich in simple sugars and lipids will promote hyperplastic changes of proximal small bowel mucosa in rodents associated with fewer enteroendocrine cells [11].

Figure 1 summarizes the role of duodenum in the regulation of insulin sensitivity and development of metabolic dysfunction [12].

Collectively, these findings provide the rationale for manipulating the duodenal mucosa to modify the absorption of nutrients in the context of metabolic disorders [12]. Various surgical and endoscopic approaches may be used to this end [13–15].

With this intriguing backset, next, we summarize and comment on a recent study by Yu et al. [16] by pinpointing novel findings, and highlighting points of strength and methodological limitations of this investigation.

Yu et al. [16] utilized male Sprague-Dawley rats fed a high-fat diet for 8 weeks to recapitulate MASLD experimentally. MASLD rats were randomly categorized into the Duodenal mucosal ablation (DMA group, n = 6) and the sham-control group (non-DMA group, n = 6). DMA was carried out using irreversible electroporation (IRE) obtained with a three-electrode ablation catheter (diameter: 2.5 mm) ending with a temperature sensor, and a square-wave pulse generator to generate a burst of electric pulses. The ablation catheter was positioned intraduodenally through pylorus. After the duodenum was ablated, the electrode catheter was withdrawn, and the abdominal incision was sutured. Postoperatively, rats were injected with buprenorphine and cefuroxime intraperitoneally and sacrificed after 2 weeks.

Three principal lines of results were documented after DMA.

Firstly, as regards the duodenum, 2 weeks after DMA, the duodenal mucosa was macroscopically healed both macroscopically and histologically, without any inflammatory, ulcerative, or stenosing complications. Moreover, although DMA was associated with significant thickening of each duodenal layer compared to the control group (P < 0.05), crypts were significantly narrower and shallower crypts and villi significantly slimmer (P < 0.05 for all comparisons) vs. non-DMA group.

Secondly, while the sham group exhibited SLD, with lipid droplets of different sizes and disorganized hepatocyte structure, liver histology was significantly improved in the DMA group. However, these histological improvements were accompanied by inconsistent variations in blood lipid values (serum total cholesterol and low-density-lipoprotein cholesterol being significantly lower whereas serum triglycerides, free fatty acids, and high-density-lipoprotein cholesterol were higher in the DMA than in the sham-control group) and statistically non-significant variations in surrogate indices of MASLD such as transaminases and total bile acids.

Thirdly, the DMA group exhibited significantly lower serum concentrations of gut hormones [glucagon-like peptide-1 (GLP-1), glucose-dependent insulinotropic polypeptide (GIP), and cholecystokinin (CCK)] with crucial metabolic functions (e.g., intake and absorption of nutrients, regulation of glucose homeostasis, secretion of pancreatic juice, and gastric and gallbladder emptying) than those in the control group (P < 0.05). Moreover, the DMA animals had significantly lower lipopolysaccharide serum levels and significantly increased duodenal expression and immunofluorescence staining intensity of GLP-1, GIP, and CCK, and higher expression of zonula occludens-1, and claudin than in the sham-control group. Collectively, these findings suggest decreased intestinal permeability after DMA.

In humans, individuals with T2D, the prototypic metabolic disorder, which is often associated with obesity, have jejunal mucosa hypertrophy, hyperplasia of enteroendocrine cells, and increased numbers of enteroendocrine cells and enterocytes compared to nondiabetic individuals; moreover, variations in glucose transporters, enteric nerves, and intestinal microbiota composition have also been reported [12]. Clinically, the best-studied endoscopic procedure is duodenal mucosa resurfacing (DMR) which is based on the principle that resurfacing the mucosal interface after endoscopic resection will reset and correct any abnormal signaling from the duodenal mucosa and will, therefore, result in improved pancreatic endocrine function and glucose tolerance owing to restored normal mucosa surface [12]. The endoscopic procedure of DMR is safe and well tolerated. However, additional studies are needed to demonstrate its validity irrespective of concurrently administered antidiabetic medications to obtain the reversal of T2D and improvement in liver histology [12]. MASLD is tightly and bi-directionally associated with T2D and it is therefore logical to speculate that DMR may also be useful in MASLD treatment irrespective of how it is implemented, namely with thermal ablation or with IRE.

The study by Yu et al. [16] has innovative findings regarding the safety and effectiveness of DMA with IRE to contrast MASLD in an experimental rat model. This is a conceptually intriguing and potentially clinically relevant research avenue. Moreover, the study by Yu et al. [16] is properly designed to illustrate the changes in gut hormones and intestinal permeability underlying this improved liver histology. However, this paper also has some methodological limitations that prompt additional studies.

One fundamental research question is whether (and to what extent) murine MASLD models faithfully recapitulate human disease. It is now clear that these models rarely show MASH with significant fibrosis (≥ F2), which typically requires protracted observations, diets including high cholesterol content, and, often, also the use of genetically engineered animals [17]. Furthermore, in the study by Yu et al. [16] only male animals have been used, which conflicts with recommendations to characterize sex differences in MASLD pathobiology and treatment [18].

Additionally, no information is provided regarding the temperature of the housing. This is important given that thermoneutral housing (i.e., a temperature where mice do not need to expend energy to maintain their core body temperature) exacerbates MASLD in mouse models, leading to more severe liver histology and even enabling female mice to develop obesity and MASLD [19].

It is also pinpointed that the procedure of DMA with IRE reported by Yu et al. [16] is extremely invasive which raises ethical concerns pertaining to the causation of unnecessary suffering to animals. Moreover, the surgical procedure involves the utilization of drugs (e.g., antibiotics and analgesics) which may potentially alter the physiology of the gastrointestinal tract by modifying the eating habits of rats owing to satiety, anorexia, vomiting, and potentially altering intestinal microbiota diversity in these animals.

All these limitations may be overcome by using animal models in which DMA with IRE may be performed endoscopically [20]. However, considering the many limitations in MASLD animal models, it will be important to study endoscopic IRE in humans as soon as sufficient evidence of safety and feasibility is obtained in preliminary studies.