The health, society, and economy of people are seriously threatened by human pathogenic viruses [1]. The possibility of viral resistance and the persistent (re-)emergence of viruses, such as the human immunodeficiency virus type 1 (HIV-1), further complicate the problem like Corona, Ebola, and Zika virus [2]. The coronavirus disease 2019 (COVID-19), which is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), spread quickly around the world [3]. Antivirals that specifically target viruses concentrate on blocking key transcription and replication enzymes, such as proteases and polymerases, or on directly inactivating viral structural proteins [4]. Usually, host-targeting antivirals and antivirals that target viruses are the most prevalent antiviral drug action mechanisms (Figure 1) [5]. Antiviral peptides usually target perturb viral membrane envelopes and restrict numerous steps of viral life cycle. The activity of antiviral peptides can be enhanced by constraining peptides into macrocycles.

In clinical studies, the repurposing of various small molecule inhibitors including favipiravir is now being studied. However, the mechanism of treatment for SARS-CoV-2 is still unrecognized [6]. Due to the possibility that peptides show more effectiveness and selectiveness than small molecule medications and will thus be better tolerated, peptide and peptide-based inhibitors are an intriguing substitute for small molecules [7]. Antiviral peptides can be a secure and efficient option for the treatment of infectious illnesses, as demonstrated by the clinical application of the anti-HIV peptide T20 [8]. Bioavailability of antiviral peptides is powerful, stable, and safe in order to be used in therapeutic settings. Peptides are extremely selective and tolerated because of their chemical makeup. Due to their relatively large size and proteolytic breakdown in the gastrointestinal (GI) tract, they may lead to less bioavailable and short half-lives [9]. Moreover, peptides can be altered to increase their activity and stability by simplifying their sequences, altering their amino acid compositions, or fusion of moieties that increase their affinities to the appropriate partner, as frequently determined by in silico modelling or scanning of amino acid [10]. The investigation of different parameters like polarity, charges, and steric effects, as well as mutagenesis studies and biomolecular simulations, are frequently used to simulate binding sites for the development of antiviral peptides [7]. Here, we report the computational-based docking study used for the development of bio-active peptides targeting SARS-CoV-2 entry. There are several microbial peptides that have a broad impact on variety of microbes and pathogens [11]. In silico and in vitro studies on anti-microbial studies against SARS-CoV-2, such as anti-microbial peptide APD3, AP00180 showed the best result with a promising docking score of –6.4 kcal/mol with nucleocapsid receptor. Some important binding interactions were observed, such as Lys85 and Arg13 with a minimum distance of 1.759 Å. Peptide AP00549 also showed the best result against SARS-CoV-2 with a docking score of –3.4 kcal/mol [12]. Rana tagoi, a species of brown frog, secretes some peptides with antiviral activity, and AR-23 is one of them. Different in vitro assay was performed on DNA and RNA containing viruses with or without envelope. Peptide AR23 was proved to be most potent against both DNA herpes simplex virus-1 (HSV-1) and RNA HSV-1 viruses [13]. Another anti-microbial peptide temporin L was collected from amphibian origin. It has strong Gram-positive and Gram-negative activity. The activity was performed in both DNA and RNA viruses. Temporin L showed significant potent inhibitory activity against herpesvirus, paramyxoviruses, influenza virus, coronaviruses, and SARS-CoV-2 [14]. Another anti-microbial peptide LL37 was evaluated by in silico screening. LL37 showed effectiveness against the disruption of SARS-CoV-2 membrane. It also decreased the viral load [15].

Cardiovascular implications are the most prominent problems in SARS-CoV-2. Severe damage includes myocardial damage, acute coronary syndrome, myocardial infarction, and cardiac arrythmia. Increased level of C-reactive protein and markers like troponine-1 may lead to severe inflammation along with hypoxic and respiratory distress [16]. The glycosylated spikes protein of SARS-CoV-2 undergoes an interaction with ACE2 receptor present in lower respiratory track, damages respiratory tissues and causes pneumonia. The reactive oxygen species (ROS) production and the upregulated cytokine level cause cell death and organ damage [17].

Main protease (M^pro) is the main target in this study with significant relevance in viral replication as it plays a crucial role in breakdown of the 12 non-structural proteins including RNA-dependent RNA polymerase (RdRp) and helicase in replication cycle. Inhibition of the M^pro disrupts the replication cycle of virus, although any inhibitor of M^pro is not reported yet [18]. Photo-thermal therapy, another invasive technique to kill virus is also used in cancer treatment. Photothermal active agents upon irradiation cause rise in vibrational energy along with heat, and the temperature above 60°C leads to cell death. In recent studies, second near infrared region (NIR-II, 1,000–1,700 nm) has been proved to be more efficient [19, 20]. The first near infrared region (NIR-I, 620–850 nm) is able to penetrate deeper tissue [21]. Photodynamic therapy (PDT) is another promising non-invasive method clinically used as antiviral therapy. It works with several photo sensitive dyes and drug agents which in excited state produce ROS that causes catalytic oxidation of the viral envelope. Peptide-based antiviral agents, especially the protease and integrase inhibitors, are not synthesized in normal synthetic method. Rather solid and solution phase peptide synthesis techniques are used, utilizing different protocols like Boc and Fmoc, and different linkers and resins as solid phase, to protect both terminals of the peptides. Both acidic and basic medium play an important role in this methodology. Some clinical trial data compiling various combination peptide therapies have been described in Table 1.

The recent antiviral marketed drugs were selected, which possess least toxicity and lowered side effects. The selected marketed drugs structures were sketched in ChemDraw and saved in Protein Data Bank (PDB) format. The structures of drugs are shown in Figure 2. Rupintrivir is a 3C cystine protease inhibitor of the picornavirus family. It also inhibits 3C-like protease of SARS-CoV-2 due to its homology. Rupintrivir inhibits M^pro cystine protease which cleaves the catalytic cystine and histidine residues [24], ATN161 (Ac-PHSCN-NH₂). It antagonises the integrin α5β1, which plays a crucial role in the inhibition of angiogenesis and metastasis of tumor. Integrin α5β1 antagonist ATN161 was derived from the synergy of the domain [Pro-His-Ser-Arg-Asn (PHSRN)], and it inhibits the tumor growth as well as ocular neovascularization [25], velpatasvir is a direct acting antiviral agent and treats chronic hepatitis C along with HIV infection. It selectively inhibits NS5A in binding domain which consists of amino acids 33–202 at RNA binding site [26], boceprevir reversibly inhibits the NS3 protease, a serine protease enzyme used in the treatment of hepatitis C virus [27]. Ombitasvir inhibits viral replication by inhibiting NS5A protein in hepatitis C viral infection [28].

To conclude, we have reported docking study of peptide based antiviral compounds for their binding mode in the catalytic domain of SARS-CoV-2 receptor. On molecular docking, the compounds have showed remarkable binding affinity with the amino acids of receptor chain A. The compounds occupied the same binding cavity as the reference compound maintaining the interactions with conserved amino acid residues essential for significant inhibitory potential, especially for compound velpatasvir with binding score of –8.2 kcal/mol. This analysis provided a mechanistic justification for the antiviral potential of the compounds under study. In vivo and in vitro studies are predicted to be desirable in the future to empirically confirm the SARS-CoV-2 inhibitory efficacy of these medicines. Significantly, the therapeutic efficacy of velpatasvir suggests that prompt treatment of individuals with mild to intermediate COVID-19 may be safe and efficient for eradicating SARS-CoV-2 more quickly and for preventing the progression and consequences of COVID-19. The fact that velpatasvir may be used to treat early infection in the outpatient context must be highlighted since doing so may be crucial for preventing the spread of infection, hospitalization, and disease progression. Further structural modifications of these compounds can be done for the generation of potent analogs. There are so many violations in drug-likeness study but as these compounds not followed oral route that’s why the drug-likeness study was less important.